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Alt a 1 ELISA kit 链格孢霉过敏原Alt a 1检测试剂盒

产品分类 > 真菌过敏原 > Alt a 1 ELISA kit 链格孢霉过敏原Alt a 1检测试剂盒

Alt a 1 ELISA kit 链格孢霉过敏原Alt a 1检测试剂盒

产品货号：EL-AA1

促销：￥12720.00

价格：￥12720.00

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产品规格：

kit

供应商：

美国Indoor

详细介绍

ELISA kit for measuring Alternaria alternata allergen, Alt a 1.
Antibodies:		2C10/3B6
Sensitivity:		0.2ng/ml
Limit of Detection:		0.4ng/ml
Assay Performance:		Inquire
Contents:		Monoclonal antibody 2C10, rAlt a 1 Standard, Biotinylated antibody 3B6; sufficient reagents for 10 microtiter plates.
Storage:		Store at 4°C.
Not Provided:		50mM carbonate/bicarbonate buffer, pH 9.6 Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T) Bovine serum albumin Streptavidin-Peroxidase 1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2
Notes:
Product Resources:		EL-AA1 Certificate of Analysis Focus on... Alt a 1 Letter on new antibodies in Alt a 1 ELISA kit
For research and commercial use in vitro: not for human in vivo or therapeutic use.
		Certificate of Analysis
Monoclonal Antibody:		2C10 (clone 2C10 G4)
Vial:		Red Cap; 100µl
Immunogen:		Alt a 1
Isotype:		Mouse IgG
Specificity:		Binds to a species specific epitope on Alternaria alternata allergen, Alt a 1.
Purification:		Produced in vitro cell culture and purified by chromatography using recombinant protein G. Single heavy and light chain bands on SDS-PAGE.
Concentration:		1mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

		Certificate of Analysis
Monoclonal Antibody:		3B6 (clone 3B6 H9)
Vial:		Brown Cap; 100µl
Immunogen:		rAlt a 1
Isotype:		Mouse IgG
Specificity:		Binds to a species specific epitope on Alternaria alternata allergen, Alt a 1.
Purification:		Produced in vitro cell culture and purified by chromatography using recombinant protein G. Single heavy and light chain bands on SDS-PAGE.
Biotinylation:		Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.

		Certificate of Analysis
Allergen Standard:		rAlt a 1
Vial:		White Cap; 400µl
Composition:		Recombinant Alt a 1 prepared in 1% BSA/50% glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4.
Concentration:		1000ng/ml Alt a 1
Calibration:		The rAlt a 1 was purified from Pichia pastoris by size exclusion HPLC. The protein content was determined by amino acid analysis.
References:
1. Vailes L, Sridhara S, Cromwell O, Weber B, Breitenbach M, Chapman M. Quantification of the major fungal allergens, Alt a 1 and Asp f 1, in commercial allergenic products. J Allergy Clin Immunol 2001;107(4):641-6. 2. Unger A, Stoger P, Simon-Nobbe B, Susani M, Crameri R, Ebner C et al. Clinical testing of recombinant allergens of the mold Alternaria alternata. Int Arch Allergy Immunol 1999;118(2-4):220-1. 3. Asturias JA, Ibarrola I, Ferrer A, Andreu C, Lopez-Pascual E, Quiralte J et al. Diagnosis of Alternaria alternata sensitization with natural and recombinant Alt a 1 allergens. J Allergy Clin Immunol 2005;115(6):1210-7. 4. Chruszcz M, Chapman MD, Osinski T, Solberg R, Demas M, Porebski PJ et al. Alternaria alternata allergen Alt a 1: A unique beta-barrel protein dimer found exclusively in fungi. J Allergy Clin Immunol 2012;130(1):241-7.
(For complete reference list see EL-AA1 Certificate of Analysis)