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Asp f 1 ELISA kit 烟曲霉过敏原Asp f 1检测试剂盒

产品分类 > 真菌过敏原 > Asp f 1 ELISA kit 烟曲霉过敏原Asp f 1检测试剂盒

Asp f 1 ELISA kit 烟曲霉过敏原Asp f 1检测试剂盒

产品货号：EL-AF1

促销：￥12720.00

价格：￥12720.00

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产品规格：

kit

供应商：

美国Indoor

详细介绍

ELISA kit for measuring Aspergillus fumigatus allergen, Asp f 1.
Sensitivity:		0.08ng/ml
Limit of Detection:		0.32ng/ml
Assay Performance:		Inquire
Contents:		Monoclonal antibody 4A6, Asp f 1 Standard, Rabbit anti-Asp f 1; sufficient reagents for 10 microtiter plates.
Storage:		Store at 4°C.
Not Provided:		50mM carbonate/bicarbonate buffer, pH 9.6 Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T) Bovine serum albumin Peroxidase conjugated Goat anti. Rabbit IgG 1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2
Notes:
Product Resources:		EL-AF1 Certificate of Analysis
For research and commercial use in vitro: not for human in vivo or therapeutic use.
		Certificate of Analysis
Monoclonal Antibody:		4A6
Vial:		Red Cap; 100µl
Immunogen:		Asp f 1
Isotype:		Mouse IgG1
Specificity:		Binds to a common epitope on Aspergillus fumigatus allergen, Asp f 1.
Purification:		Produced in tissue culture and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.
Concentration:		2mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

		Certificate of Analysis
Antibody:		Polyclonal antibody raised against partially purified Asp f 1 (1,2).
Vial:		Brown Cap; 100µl
Specificity:		The pAb contains IgG antibodies to Asp f 1 as well as IgG antibodies to other Aspergillus allergens.
Activity:		The pAb is in phosphate buffered saline containing 1%BSA 50%glycerol PBS, pH 7.4. The pAb has been 0.22µm filtered and should be diluted 1/1000 for use in Asp f 1 ELISA.

		Certificate of Analysis
Allergen Standard:		rAsp f 1
Vial:		White Cap; 400µl
Composition:		Recombinant Asp f 1 prepared in 1% BSA/50% glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4
Concentration:		400ng/ml rAsp f 1
Concentration:		The Asp f 1 concentration was determined by amino acid analysis. Asp f 1 and Asp r 1 (mitogillin) are 95% homologous (differing by 4 amino acid residues) and are antigenically and functionally indistinguishable (1).
References:
1. Arruda, LK, Platts-Mills, TAE, Fox, JW and Chapman, MD (1990). Aspergillus fumigatus allergen I, a major IgE binding protein, is a member of the mitogillin family of cytotoxins. J. Exp. Med., 172:1529-1532. 2. Arruda LK, Platts-Mills TAE, Longbottom JL, Chapman MD (1992). Aspergillus fumigatus: Identification of 16kd, 18kd and 45kd antigens recognized by human IgG and IgE antibodies and murine monoclonal antibodies. J. Allergy Clin. Immunol. 89:1166-1176. 3. Arruda LK, Mann BJ, Chapman MD (1992). Selective expression of a major allergen and cytotoxin, Asp f 1, in Aspergillus fumigatus. J. Immunol. 149:3354- 3359. 4. Sporik RB, Arruda LK, Woodfolk J, Chapman MD, Platts-Mills TAE (1993). Environmental exposure to Aspergillus fumigatus allergen 1 (Asp f 1). Clin. Exp. Allergy 23:326-331. 5. El-Dahr JM, Fink R, Selden R, Arruda LK, Platts-Mills TAE, Heymann PW (1994). Development of immune responses to Aspergillus at an early age in children with cystic fibrosis. Am. J. Respir. Crit. Care Med. 150:1513-1518. 6. Vailes LD, Sridhara S, Cromwell O, Weber B, Breitenbach M, Chapman MD (2001). Quantitation of the major fungal allergens, Alt a 1 and Asp f 1, in commercial allergenic products. J. Allergy Clin. Immunol. 107:641-646. 7. Chapman MD (2006). Challenges associated with indoor moulds: Health effects, immune response and exposure assessment. Medical Mycology. 44:S29-S32.
(For complete reference list see EL-AF1 Certificate of Analysis)