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产品分类 > 皮屑毛发羽毛过敏原 > Can f 1 ELISA kit 狗过敏原Can f1检测试剂盒

Can f 1 ELISA kit 狗过敏原Can f1检测试剂盒

产品货号:EL-CF1
促销: 12720.00
价格: 12720.00

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kit
美国Indoor

ELISA kit for measuring Canis familiaris allergen, Can f 1.

Antibodies:10D4/pAb

Sensitivity:0.5ng/ml

Limit of Detection:0.5ng/ml

Assay Performance:Intra-assay CV% of 6.0; Inter-assay CV% of 9.5

Contents:

Monoclonal antibody 10D4, Universal Allergen

Standard, Polyclonal Rabbit anti-Can f 1; sufficient

reagents for 10 microtiter plates.

Storage:The ELISA kit should be stored at 4°C.

Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6

Phosphate buffered saline, pH 7.4, containing 0.05%

Tween 20 (PBS-T)

Bovine serum albumin

Peroxidase conjugated Goat anti. Rabbit IgG

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:
Product Resources:EL-CF1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.


Certificate of Analysis

Monoclonal Antibody:10D4 (clone 10D4 C4 A10)

Vial:Red Cap; 100µl

Immunogen:Can f 1

Isotype:Mouse IgG2ak

Specificity:Binds to an epitope on dog Canis familiarisallergen,

Can f 1.
Purification:Produced in ascites and purified by affinity

chromatography using Protein A. Single heavy and light

chain bands on SDS-PAGE.

Concentration:1mg/ml in phosphate buffered saline, pH 7.4. Based on

A280 for IgG (1.42=1mg/ml), 0.22μm filtered,

preservative free.



Certificate of Analysis

Antibody:Rabbit polyclonal antiserum

Immunogen:Dog fur extract

Vial:Brown Cap; 100µl

Specificity:The antiserum contains IgG antibodies to dog Canis

familiaris
 allergen, Can f 1, as well as IgG antibodies to

other dog allergens.

Activity:Titrated for use in ELISA at 1/1000 dilution. Prepared in

1%BSA/ 50%glycerol/ PBS, pH 7.4., 0.22μm filtered,

preservative free.



Certificate of Analysis

Allergen Standard:Universal Allergen Standard

Vial:White Cap; 400µl

Composition:A formulation of eight purified natural allergens

prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration/Calibration:

Universal Allergen StandardProtein MeasurementConcentration (ng/ml)
Der p 1Amino-acid analysis

2500
Der f 1Amino-acid analysis

2500
Der p 2Amino-acid analysis

1000
Fel d 1Amino-acid analysis

1000
Can f 1Amino-acid analysis

2500
Rat n 1Amino-acid analysis

1000
Mus m 1Amino-acid analysis

250
Bla g 2Amino-acid analysis

2500
References:


1.  Larsen JN, Ford A, Gjesing B, et al. The collaborative study of the international standard of dog, Canis domesticus, hair/dander extract. J Allergy Clin Immunol 1988; 82:318-330.
2.  DeGroot H, Goei K, Van Sicter P, et al. Affinity purification of a major and minor allergen from dog extract: Serologic activity of affinity-purified Can f 1 and Can f 1-depleted extract. J Allergy Clin Immunol 1991; 87:1056-61.
3.  Schou C, Hansen G, Linter T, et al. Assay for the major dog allergen, Can f 1 investigation of house dust samples and commercial dog extracts. J Allergy Clin Immunol 1992; 88:847-53.
4.  Ingram JM, Sporik R, Rose G, Honsiger R, Chapman MD, Platts-Mills TAE. Quantitative assessment of exposure to dog (Can f 1) and cat (Fel d 1) allergens: Relationship to sensitization and asthma among children living in Los Alamos, New Mexico. J Allergy Clin Immunol 1995; 96:449-456.
5.  Sporik RB, Ingram JM, Price W, Sussman JH, Honsiger RW, Platts-Mills TAE. Association of asthma with serum IgE and skin test reactivity to allergens among children living at high altitude: Tickling the dragon’s breath. Amer J Resp Crit Care Med 1995;151:1388-1392.
(For complete reference list see EL-CF1 Certificate of Analysis)