Recombinant Can f 1 重组狗过敏原Can f1

Purified from Pichia pastoris culture using His-Trap 

chromatography.  Purity >95% on silver 

stained SDS-PAGE.  

See Product Insert. 

Preservative and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22µm filter.

(1) rCan f 1 presents as a 19kD unglycosylated and a 23 kD glycosylated monomer. In addition, a dimer around 40kD is visible under non-reducing conditions.

(2) rCan f 1 has a C-terminal 6xHis-tag

1. Konieczny A, et al.  The major dog allergens, Can f 1 and Can f 2, are salivary lipocalin proteins: cloning and immunological characterization of the recombinant forms.  Immunology 1997;92:577-86.

2. Saarelainen S et al. Assessment of recombinant dog allergens Can f 1 and Can f 2 for the diagnosis of dog allergy. Clin Exp Allergy. 2004;34(10):1576-82.