Bla g 2 ELISA kit (EL-BG2) 德国小蠊过敏原Bla g2检测试剂盒

Antibodies:

7C11/pAb

Sensitivity:

0.5ng/ml

Limit of Detection:

0.5ng/ml

Assay Performance:

Inquire

Contents:

Monoclonal antibody 7C11, Universal   Allergen Standard, Polyclonal Rabbit anti-Bla g 2; sufficient reagents for 10   microtiter plates.

Storage:

Store at 4°C

Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6
  Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
  Bovine serum albumin
  Peroxidase conjugated Goat anti. Rabbit IgG
  1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:

Product Resources:

EL-BG2 Certificate of Analysis

For research and commercial use in vitro: not for   human in vivo or therapeutic use.

Certificate of Analysis

Monoclonal Antibody:

7C11 (Clone 7C11 C2 A6)

Vial:

Red Cap; 100µl

Immunogen:

Bla g 2

Isotype:

Mouse IgG1

Specificity:

Binds to an epitope on cockroach Blattella   germanica allergen, Bla g 2.

Purification:

Produced in ascites and purified by ammonium sulphate   precipitation and affinity chromatography using Protein A. Single heavy and   light chain bands on SDS-PAGE.

Concentration:

1mg/ml in phosphate buffered saline, pH 7.4, based on   A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

Certificate of Analysis

Antibody:

Rabbit polyclonal antiserum

Vial:

Brown Cap; 100µl

Immunogen:

Recombinant Bla g 2

Specificity:

The antiserum contains IgG antibodies to cockroach Blattella   germanica allergen, Bla g 2 and does not react with Bla g 1.

Activity:

Titrated for use in ELISA at 1/1000 dilution. Prepared   in 1%BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.

Certificate of Analysis

Allergen Standard:

Universal Allergen Standard

Vial:

White Cap; 400µl/vial

Composition:

A formulation of eight purified natural allergens   prepared in 1% BSA/50% glycerol/PBS, pH 7.4.

Concentration/Calibration:

Universal Allergen Standard

Protein Measurement

Concentration (ng/ml)

Der p 1

Amino-acid analysis

2500

Der f 1

Amino-acid analysis

2500

Der p 2

Amino-acid analysis

1000

Fel d 1

Amino-acid analysis

1000

Can f 1

Amino-acid analysis

2500

Rat n 1

Amino-acid analysis

1000

Mus m 1

Amino-acid analysis

250

Bla g 2

Amino-acid analysis

2500

References:

1.  Pollart SM, Smith TF, Morris E, Gelber LE,   Platts-Mills TAE, Chapman MD. Environmental exposure to cockroach allergens:   analysis with monoclonal Antibody based enzyme immunoassays. J Allergy Clin   Immunol 87:505- 510, 1991.

2.  Gelber LE, Seltzer LH, Bouzoukis JK, Pollart   SM, Chapman MD, Platts-Mills TAE. Sensitization ad exposure to indoor   allergens as risk factors for asthma among patients presenting to hospital.   Amer Rev Respir Dis 147:573-578, 1993.

3.  Rosenstreich DL, Eggleston P, Kattan M, Baker   D, Slavin RG, Gergen P, Mitchell H, McNiff-Mortimer K, Lynn H, Ownby D,   Malveaux F. The role of cockroach allergy and exposure to cockroach allergen   in causing morbidity among inner-city children with asthma. New Eng J Med   336:1356-1363, 1997.

4.  Arruda LK, Vailes LD, Ferriani VP, Santos AB,   Pomés A, Chapman MD. Cockroach allergens and asthma. J Allergy Clin Immunol   107:419-428, 2001.

5. Pomés A, Vailes LD, Helm RM, Chapman MD. IgE   reactivity of tandem repeats derived from cockroach allergen, Bla g 1. Eur J   Biochem 269:3086-3092, 2002.

6.  Nalyanya G, Gore JC, Linker HM, Schal C.   German cockroach allergen levels in North Carolina schools: comparison of   integrated pest management and conventional cockroach control. J Med Entomol   46:420-427, 2009.

(For complete reference list see EL-BG2 Certificate of Analysis)