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产品分类 > 自免疾病检测原料 > Glycyl-tRNA Synthetase (EJ)

Glycyl-tRNA Synthetase (EJ)

产品货号:11100/11101
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0.1mg 1mg
德国Diarect

Product Number: 11100
Description:
Human Glycyl-tRNA Synthetase (EC 6.1.1.14; EJ). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays.
Immunological function:
Binds IgG-type human auto-antibodies.
Origin:
Recombinant. Expressed by recombinant baculovirus (Autographa californica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insect cells.
Expression construct:
Full-length cDNA coding for the human Glycyl-tRNA Synthetase fused to a deca-histidine purification tag.
Biochemical tests:
SDS-PAGE (purity > 80%); Western blot with i: anti-EJ positive sera; ii: monoclonal anti-His-tag antibody.
Calculated molecular weight:
78,902 Dalton
Calculated isoelectric point:
pH 6.17
Immunological tests/Functionality: Immunodot test with positive/negative sera.
Recommended buffer/storage and handling conditions:
Recommendations for storage buffer: ionic strength between 100 and 200 mM salt, neutral to slightly alkaline pH and 20% glycerol as cryoprotective agent. Storage temperature:
–70° to –80° C. Repeated freeze/thaw cycles should be avoided.
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