上海博升生物科技有限公司 欢迎您!   邮箱登录  网站地图  旧站入口

产品分类 > 过敏疾病检测原料 > Mus m 1 ELISA kit (EL-MM1)

Mus m 1 ELISA kit (EL-MM1)

产品货号:EL-MM1
促销: 12720.00
价格: 12720.00

分享

kit
美国Indoor

ELISA kit for measuring Mouse Urinary Protein (MUP) allergen, Mus m 1.

Antibodies:pAb/pAb

Sensitivity:0.05ng/ml

Limit of Detection:0.2ng/ml

Assay Performance:Intra-assay CV% of 6.1; Inter-assay CV% of 14.4

Contents:

Rabbit anti. Mus m 1, Universal Allergen Standard, Biotinylated Rabbit anti. Mus m 1; sufficient reagents for 10 microtiter plates.

Storage:Store at 4°C

Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6

Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)

Bovine serum albumin

Streptavidin-Peroxidase

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:


Product Resources:EL-MM1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.




Certificate of Analysis

Polyclonal Antibody:Rabbit polyclonal antiserum

Vial:Red Cap; 100µl

Immunogen:Mus m 1

Isotype:Multiple

Specificity:Binds to an epitope on mouse Mus musculusurinary protein allergen, Mus m 1.

Purification:Affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.

Concentration:1.6mg/ml in phosphate buffered saline, pH 7.4.  Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.



Certificate of Analysis

Polyclonal Antibody:Rabbit polyclonal antiserum

Vial:Brown Cap; 100µl

Immunogen:Mus m 1

Isotype:Multiple

Specificity:Binds to an epitope on mouse Mus musculusurinary protein allergen, Mus m 1.

Purification:Affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.

Biotinylation:Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.



Certificate of Analysis

Allergen Standard:Universal Allergen Standard

Vial:White Cap; 400µl

Composition:A formulation of eight purified natural allergens prepared in 1% BSA/50% glycerol/PBS, pH 7.4

Concentration/Calibration:

Universal Allergen Standard

Protein Measurement

Concentration (ng/ml)

Der p 1

Amino-acid analysis

2500

Der f 1

Amino-acid analysis

2500

Der p 2

Amino-acid analysis

1000

Fel d 1

Amino-acid analysis

1000

Can f 1

Amino-acid analysis

2500

Rat n 1

Amino-acid analysis

1000

Mus m 1

Amino-acid analysis

250

Bla g 2

Amino-acid analysis

2500

References: 

1. Phipatanakul W, Eggleston PA, Wright EC, Wood RA.  Mouse allergen. I. The prevalence of mouse allergen in inner-city homes. The National Cooperative Inner-City Asthma Study.  J Allergy Clin Immunol. 2000;106:1070-4. 

2. Phipatanakul W, Eggleston PA, Wright EC, Wood RA.  Mouse allergen. II. The relationship of mouse allergen exposure to mouse sensitization and asthma morbidity in inner-city children with asthma.  J Allergy Clin Immunol. 2000;106:1075-80.

3. Permaul P, Hoffman E, Fu C, Sheehan W, Baxi S, Gaffin J, Lane J, Bailey A, King E, Chapman M, Gold D, Phipatanakul W.  Allergens in urban schools and homes of children with asthma.  Pediatr Allergy Immunol. 2012;23:543-9.

4.  Ahluwalia SK, Peng RD, Breysse PN, Diette GB, Curtin-Brosnan J, Aloe C, Matsui EC.  Mouse allergen is the major allergen of public health relevance in Baltimore City.  J Allergy Clin Immunol. 2013;132:830-5.

5.  Ownby DR.  Will the real inner-city allergen please stand up?  J Allergy Clin Immunol. 2013;132:836-7.

(For complete reference list see EL-MM1 Certificate of Analysis)