TIF1 gamma

Product Number: 11000
Description:
Human transcriptional intermediary factor 1 gamma (EC 6.3.2.- ; TIF1 gamma). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays.
Immunological function:
Binds IgG-type human auto-antibodies.
Origin:
Recombinant. Expressed by recombinant baculovirus (Autographa californica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insect cells.
Expression construct:
Full-length cDNA coding for the human transcriptional intermediary factor 1 gamma fused to a deca-histidine purification tag.
Biochemical tests:
SDS-PAGE (purity > 80%); Western blot with i: anti-TIF1 gamma positive sera; ii: monoclonal anti-His-tag antibody.
Calculated molecular weight:
124,041 Dalton
Calculated isoelectric point:
pH 6.38
Immunological tests/Functionality: Immunodot test with positive/negative sera panels.
Recommended buffer/storage and handling conditions:
Recommendations for storage buffer: ionic strength between 400 and 600 mM salt, neutral to slightly alkaline pH and 6M Urea.

Storage temperature:
–70° to –80° C. Repeated freeze/thaw cycles should be avoided