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产品分类 > 自身免疫甲状腺疾病 > Thyroid Peroxidase (TPO)/甲状腺过氧化物酶

Thyroid Peroxidase (TPO)/甲状腺过氧化物酶

产品货号:12100 /12101
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0.1mg 1mg
德国Diarect

Thyroid Peroxidase (TPO)

Diseases:
Autoimmune Thyroiditis (e.g. Graves Disease, Hashimoto Thyroiditis)
 

Thyroid peroxidase (TPO) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. Its identity with the formerly so-called microsomal antigen has been shown several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx. 100 kDa molecular weight. The hemoprotein TPO plays a key role in thyroid hormone biosynthesis by catalyzing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin (TG) to form precursors of the thyroid hormones T4 and T3.

Thyroid peroxidase (TPO) is expressed by DIARECT in the baculovirus expression system. DIARECT produces a truncated soluble TPO molecule, which consists of the epitope-bearing extracytoplasmic domain. The recombinant production of an engineered TPO antigen eliminates the purity problems of the classical microsomal antigen preparations, which are inevitably contaminated with thyroglobulin, one of the other major thyroid autoantigens.

TPO antibodies, the hallmark of human autoimmune thyroid disease, are of IgG class. TPO antibodies occur with a prevalence of about 90% in patients with Hashimoto thyroiditis and in patients with Graves Disease at a lower prevalence of 70-95%.

 

    相关疾病:自身免疫性甲状腺疾病(桥本氏甲状腺炎,Grave’s病)

    甲状腺过氧化物酶是自身免疫性甲状腺疾病中重要的自身抗原标靶之一。被称为微粒体抗原的甲状腺过氧化物酶早在几年前已被证实其存在。作为一个内膜糖蛋白,它位于甲状腺滤泡上皮细胞的顶端质膜上,含有两个相同的亚基,分子量约为100kDa。血红素蛋白TPO在甲状腺激素合成中扮演着重要的角色,它不但催化酪氨酸残基的碘化,而且在免疫球蛋白到甲状腺激素T3和T4的初期形式中碘化酪氨酸残基的偶联。

DIARECT在杆状病毒表达系统中表达TPO,DIARECT生产的TPO分子是包含胞质外域抗原表位的截短的可溶物。重组TPO抗原根除了经典的微粒体抗原制备过程中的纯度问题,不会有甲状腺球蛋白的污染。TPO抗体是人类自身免疫甲状腺疾病的标志,属于IgG。90%的桥本氏甲状腺炎,以及

70-95%的Graves病是由TPO自身抗体造成的。