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产品分类 > 乳糜泻(麸质过敏症) > Tissue Transglutaminase (tTG; Ec)/组织转谷氨酰胺酶(在大肠杆菌中表达)

Tissue Transglutaminase (tTG; Ec)/组织转谷氨酰胺酶(在大肠杆菌中表达)

产品货号:14400/14401
促销: 0.00

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0.1mg 1mg
德国Diarect

Tissue Transglutaminase (tTG; Ec)

Diseases:
Celiac Disease
 

Celiac disease is an enteropathy that is characterized by intestinal lesions of variable severity. Tissue-type transglutaminase (tTG) is believed to be the predominant autoantigen for celiac disease, and the corresponding autoantibodies show higher sensitivity and specificity than anti-gliadin antibodies. Highly pure recombinant human tTG is now available to replace the traditionally used tTG fraction from guinea pig.

Tissue-type transglutaminase antigens from DIARECT have been specifically modified for improved handling: exchange of an active site amino acid eliminates the protein cross-linking activity of the enzyme, while maintaining the native three-dimensional structure and the enzymes secondary GTPase activity. This engineering assures reproducible properties of the antigen preparations by eliminating variable and ill-defined covalent aggregates of tTG antigen and host-cell proteins.

Two recombinant human tissue transglutaminase products are available from DIARECT, expressed in baculovirus / Sf9 insect cells and E.coli respectively. 

 

相关疾病:麸质过敏症麸质过敏症又叫做乳糜泻,是指在遗传性的易感个体中,一种在小肠炎症条件下导致的对摄入一定的饮食谷蛋白——麦醇溶蛋白过敏症。该疾病的发病机制来自环境的,遗传的和免疫的因素的相互作用的结果。对该种病人唯一的治疗办法就是终生禁止含麦醇溶蛋白食物的饮用,这样能使肠黏液得到恢复。组织型谷氨酰胺转移酶做为抗原可检测出自身抗体,促使发病机制得到更好的理解并且促进了重要的血清学测试的发展。麸质过敏症是一种以不同程度肠损伤为特征的肠病。组织型谷氨酰胺转移酶(tTG)被认为是麸质过敏症的主要抗原,其相应的抗体比其他抗麦胶蛋白抗体具有更高的灵敏性和特异性。高纯度的重组人tTG现可用来替换传统的荷兰猪tTG片段。DIARECT生产的tTG抗原特别改进了工艺:替换掉了一个氨基酸活性位点以消除酶的交叉反应活性,同时保持了蛋白的天然三维结构和酶的次级GTP酶活性。该工程通过消除可变的不清楚的tTG抗原和宿主蛋白的共价聚合,保证了抗原的可再制性。

DIARECT生产的两种tTG抗原分别在昆虫细胞和大肠杆菌表达系统中表达。