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产品分类 > 花粉过敏原 > Phl p 5 ELISA kit 猫尾草过敏原Phl p 5 检测试剂盒

Phl p 5 ELISA kit 猫尾草过敏原Phl p 5 检测试剂盒

产品货号:EL-PP5
促销: 12720.00
价格: 12720.00

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kit
美国Indoor
ELISA kit for measuring Phleum pratense allergen, Phl p 5.

Antibodies:1D11/Bo1

Sensitivity:1ng/ml

Limit of Detection:4ng/ml

Assay Performance:Inquire

Contents:

Monoclonal antibody 1D11, Phl p 5 Standard, Biotinylated antibody Bo1; sufficient reagents for 10 plates.

Storage:Store at 4°C.

Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6

Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)

Bovine Serum Albumin

Streptavidin-Peroxidase

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:
Product Resources:EL-PP5 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.




Certificate of Analysis

Monoclonal Antibody:1D11 (clone 1D11 C8)

Vial:Red Cap; 100µl

Immunogen:Timothy grass pollen extract

Isotype:Mouse IgG1

Specificity:

Binds to species specific epitope present on timothy grass pollen allergen, Phl p 5a & b.

Purification:Produced in tissue culture and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. 

Concentration:1.5mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free. 



Certificate of Analysis

Monoclonal Antibody:Bo1

Vial:Brown Cap; 100µl

Immunogen:Phl p 5

Isotype:Mouse IgG1

Specificity:

Binds to species specific epitope present on timothy grass pollen allergen, Phl p 5a & b.

Purification:Produced in tissue culture and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

Biotinylation:Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, 0.22µm filtered, preservative free. 



Certificate of Analysis

Allergen Standard:rPhl p 5a

Vial:White Cap; 400µl

Composition:Recombinant Phl p 5 prepared in 1% BSA/50 glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4.

Concentration:5000ng/ml


Calibration:The rPhl p 5a was from E. coli and purified by conventional biochemical methods. Validity of the rPhl p 5 standard was confirmed by comparison with the European Pharmacopoeia reference CRS (Y0001566),

recombinant major allergen rPhl p 5 containing 8.56 μg of rPhl p 5a per vial. 

References:

  1. Müller WD, Diener C, Jung K, Jäger L. Antigens of Timothy and othergrass pollen extracts identified by monoclonal antibodies. AllergolImmunopathol (Madr). 1988;16:315-20. 

  2. Fahlbusch B, Müller WD, Diener CH, Jäger L. Detection of crossreactivedeterminants in grass pollen extracts using monoclonal antibodiesagainst group IV and group V allergens. Clin Exp Allergy. 1993;23:51-60. 

  3. Petersen A, Becker WM, Schlaak M. Epitope analysis of isoforms of themajor allergen Phl p V by fingerprinting and microsequencing. Clin ExpAllergy. 1994;24:250-6. 

  4. S. Vieths, D. Barber, M. Chapman, A. Costanzo, A. Daas, H. Fiebig, K.M.Hanschmann, M. Hrabina, S. Kaul, A. Ledesma, P. Moingeon, G. Reese,C. Schörner, R. van Ree, B. Weber, K.H. Buchheit. Establishment ofrecombinant major allergens Bet v 1 and Phl p 5a as Ph. Eur. Referencestandards and validation of ELISA methods for their measurement.Pharmeuropa Bio&SN October 2012; 118-13. 

(For complete reference list see EL-PP5 Certificate of Analysis)