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Phl p 5 ELISA kit 猫尾草过敏原Phl p 5 检测试剂盒

产品分类 > 花粉过敏原 > Phl p 5 ELISA kit 猫尾草过敏原Phl p 5 检测试剂盒

Phl p 5 ELISA kit 猫尾草过敏原Phl p 5 检测试剂盒

产品货号：EL-PP5

促销：￥12720.00

价格：￥12720.00

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产品规格：

kit

供应商：

美国Indoor

详细介绍

ELISA kit for measuring Phleum pratense allergen, Phl p 5.
Antibodies:		1D11/Bo1
Sensitivity:		1ng/ml
Limit of Detection:		4ng/ml
Assay Performance:		Inquire
Contents:		Monoclonal antibody 1D11, Phl p 5 Standard, Biotinylated antibody Bo1; sufficient reagents for 10 plates.
Storage:		Store at 4°C.
Not Provided:		50mM Carbonate/Bicarbonate buffer, pH 9.6 Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T) Bovine Serum Albumin Streptavidin-Peroxidase 1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2
Notes:
Product Resources:		EL-PP5 Certificate of Analysis
For research and commercial use in vitro: not for human in vivo or therapeutic use.

		Certificate of Analysis
Monoclonal Antibody:		1D11 (clone 1D11 C8)
Vial:		Red Cap; 100µl
Immunogen:		Timothy grass pollen extract
Isotype:		Mouse IgG1
Specificity:		Binds to species specific epitope present on timothy grass pollen allergen, Phl p 5a & b.
Purification:		Produced in tissue culture and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Concentration:		1.5mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

		Certificate of Analysis
Monoclonal Antibody:		Bo1
Vial:		Brown Cap; 100µl
Immunogen:		Phl p 5
Isotype:		Mouse IgG1
Specificity:		Binds to species specific epitope present on timothy grass pollen allergen, Phl p 5a & b.
Purification:		Produced in tissue culture and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation:		Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, 0.22µm filtered, preservative free.

		Certificate of Analysis
Allergen Standard:		rPhl p 5a
Vial:		White Cap; 400µl
Composition:		Recombinant Phl p 5 prepared in 1% BSA/50 glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4.
Concentration:		5000ng/ml
Calibration:		The rPhl p 5a was from E. coli and purified by conventional biochemical methods. Validity of the rPhl p 5 standard was confirmed by comparison with the European Pharmacopoeia reference CRS (Y0001566), recombinant major allergen rPhl p 5 containing 8.56 μg of rPhl p 5a per vial.
References: Müller WD, Diener C, Jung K, Jäger L. Antigens of Timothy and othergrass pollen extracts identified by monoclonal antibodies. AllergolImmunopathol (Madr). 1988;16:315-20. Fahlbusch B, Müller WD, Diener CH, Jäger L. Detection of crossreactivedeterminants in grass pollen extracts using monoclonal antibodiesagainst group IV and group V allergens. Clin Exp Allergy. 1993;23:51-60. Petersen A, Becker WM, Schlaak M. Epitope analysis of isoforms of themajor allergen Phl p V by fingerprinting and microsequencing. Clin ExpAllergy. 1994;24:250-6. S. Vieths, D. Barber, M. Chapman, A. Costanzo, A. Daas, H. Fiebig, K.M.Hanschmann, M. Hrabina, S. Kaul, A. Ledesma, P. Moingeon, G. Reese,C. Schörner, R. van Ree, B. Weber, K.H. Buchheit. Establishment ofrecombinant major allergens Bet v 1 and Phl p 5a as Ph. Eur. Referencestandards and validation of ELISA methods for their measurement.Pharmeuropa Bio&SN October 2012; 118-13.
(For complete reference list see EL-PP5 Certificate of Analysis)