ELISA kit for measuring S. chartarum antigen. | ||||
Sensitivity: | 2U/ml | |||
Limit of Detection: | 31U/ml | |||
Assay Performance: | Inquire | |||
Contents: | Monoclonal antibody 3F10, Stachybotrys chartarum Standard, Biotinylated antibody 3F10; sufficient reagents for 10 microtiter plates. | |||
Storage: | Store at 4°C. | |||
Not Provided: | 50mM carbonate/bicarbonate buffer, pH9.6 Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T) Bovine serum albumin Streptavidin-Peroxidase 1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2 | |||
Notes: | ||||
Product Resources: | EL-SCY Certificate of Analysis | |||
For research and commercial use in vitro: not for human in vivo or therapeutic use. | ||||
Certificate of Analysis | ||||
Monoclonal Antibody: | 3F10 (clone 3F10 A5 B10) | |||
Vial: | Red Cap; 100µl | |||
Immunogen: | Stachybotrys chartarum extract | |||
Isotype: | Mouse IgG1 | |||
Specificity: | Binds to a species specific epitope of Stachybotrys chartarum antigen. | |||
Purification: | Produced in tissue culture and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | |||
Concentration: | 1mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free. | |||
Certificate of Analysis | ||||
Monoclonal Antibody: | 3F10 (clone 3F10 A5 B10) | |||
Vial: | Brown Cap; 100µl | |||
Immunogen: | Stachybotrys chartarum extract | |||
Isotype: | Mouse IgG1 | |||
Specificity: | Binds to a species specific epitope of Stachybotrys chartarum antigen. | |||
Purification: | Produced in tissue culture and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | |||
Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free. | |||
Certificate of Analysis | ||||
Antigen Standard: | Stachybotrys chartarum | |||
Vial: | White Cap; 400µl | |||
Composition: | Stachybotrys chartarum culture extract prepared in 1% BSA/50% glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4. | |||
Concentration: | 10,000U/ml SchY | |||
Calibration: | The concentration of the extract was measured using a total protein assay. The extract is calibrated in arbitrary units where 1 unit of SchY equals approximately 1 nanogram of total protein. |