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Ara h 1 ELISA kit 花生过敏原Ara h1检测试剂盒

产品分类 > 食物过敏原 > Ara h 1 ELISA kit 花生过敏原Ara h1检测试剂盒

Ara h 1 ELISA kit 花生过敏原Ara h1检测试剂盒

产品货号：EL-AH1

促销：￥12720.00

价格：￥12720.00

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产品规格：

kit

供应商：

美国Indoor

详细介绍

ELISA kit for measuring Arachis hypogaea allergen, Ara h 1.
Antibodies:		2C12/2F7
Sensitivity:		4ng/ml
Limit of Detection:		31.5ng/ml
Assay Performance:		Intra-assay CV% of 3.1; Inter-assay CV% of 11.8
Contents:		Monoclonal antibody 2C12, Ara h 1 Allergen Standard, Biotinylated antibody 2F7; sufficient reagents for 10 microtiter plates.
Storage:		Store antibodies at 4°C; store ST-AH1 at -20°C upon arrival (standard has 3 month shelf life).
Not Provided:		50mM carbonate/bicarbonate buffer, pH 9.6 Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS- T) Bovine serum albumin Streptavidin-Peroxidase 1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2
Notes:
Product Resources:		EL-AH1 Certificate of Analysis
For research and commercial use in vitro: not for human in vivo or therapeutic use.
		Certificate of Analysis
Monoclonal Antibody:		2C12 (clone 2C12 A11 A3)
Vial:		Red Cap; 100µl
Immunogen:		Ara h 1
Isotype:		Mouse IgG₁
Specificity:		Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 1.
Purification:		Produced in vitro by BioVectra dcl bioreactors and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.
Concentration:		2.7 mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

		Certificate of Analysis
Monoclonal Antibody:		2F7 (clone 2F7 C12 D10)
Vial:		Brown Cap; 100µl
Immunogen:		Ara h 1
Isotype:		Mouse IgG₁
Specificity:		Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 1.
Purification:		Produced in ascites and purified by affinity chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.
Biotinylation:		Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

		Certificate of Analysis
Allergen Standard:		nAra h 1
Vial:		White Cap; 400µl
Composition:		Naturally purified Ara h 1 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration:		20,000ng/ml Ara h1
Calibration:		The Ara h 1 concentration of the purified Ara h 1 was determined by OD₂₈₀.
Stability/Storage:		Please store the standard at -20ºC (+/- 5°C).
References: 1. Pomés A, Helm RM, Bannon GA, Burks AW, Tsay A, Chapman MD. Monitoring peanut allergen in food products by measuring Ara h 1. J Allergy Clin Immunol 2003;111:640-5. 2. Pomés A, Vinton R, Chapman MD. Peanut allergen (Ara h 1) detection in foods containing chocolate. J Food Prot. 2004 Apr:67 (4):793-8. 3. Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6. 4. Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24.
(For complete reference list see EL-AH1 Certificate of Analysis)