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产品分类 > 食物过敏原 > Ara h 1 ELISA kit 花生过敏原Ara h1检测试剂盒

Ara h 1 ELISA kit 花生过敏原Ara h1检测试剂盒

产品货号:EL-AH1
促销: 12720.00
价格: 12720.00

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kit
美国Indoor
ELISA kit for measuring Arachis hypogaea allergen, Ara h 1.

Antibodies:2C12/2F7

Sensitivity:4ng/ml

Limit of Detection:31.5ng/ml

Assay Performance:Intra-assay CV% of 3.1; Inter-assay CV% of 11.8

Contents:

Monoclonal antibody 2C12, Ara h 1 Allergen Standard, Biotinylated

antibody 2F7; sufficient reagents for 10 microtiter plates.

Storage:Store antibodies at 4°C; store ST-AH1 at -20°C upon arrival (standard

has 3 month shelf life).
Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6

Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-
T)

Bovine serum albumin

Streptavidin-Peroxidase

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:


Product Resources:EL-AH1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.


Certificate of Analysis

Monoclonal Antibody:2C12 (clone 2C12 A11 A3)

Vial:Red Cap; 100µl

Immunogen:Ara h 1

Isotype:Mouse IgG1

Specificity:Binds to species specific epitope present on Arachis

hypogaea
 allergen, Ara h 1.

Purification:Produced in vitro by BioVectra dcl bioreactors and purified by

chromatography using Protein G. Single heavy and light chain bands

on SDS-PAGE.

Concentration:2.7 mg/ml in phosphate buffered saline, pH 7.4.  Based on A280 for

IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.



Certificate of Analysis

Monoclonal Antibody:2F7 (clone 2F7 C12 D10)

Vial:Brown Cap; 100µl

Immunogen:Ara h 1

Isotype:Mouse IgG1

Specificity:Binds to species specific epitope present on Arachis

hypogaea
 allergen, Ara h 1.
Purification:Produced in ascites and purified by affinity chromatography using

Protein G. Single heavy and light chain bands on SDS-PAGE.

Biotinylation:Biotinylated and titrated for use in ELISA at 1/1000 dilution.

Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered,

preservative free.


Certificate of Analysis

Allergen Standard:nAra h 1

Vial:White Cap; 400µl

Composition:Naturally purified Ara h 1 prepared in 1% BSA/50% glycerol/PBS,

pH 7.4.
Concentration:20,000ng/ml Ara h1

Calibration:The Ara h 1 concentration of the purified Ara h 1 was determined by

OD280.
Stability/Storage:Please store the standard at -20ºC (+/- 5°C).

References:

1.  Pomés A, Helm RM, Bannon GA, Burks AW, Tsay A, Chapman MD. Monitoring peanut allergen in food products by measuring Ara h 1. J Allergy Clin Immunol 2003;111:640-5.

2.  Pomés A, Vinton R, Chapman MD. Peanut allergen (Ara h 1) detection in foods containing chocolate. J Food Prot. 2004 Apr:67 (4):793-8.

3.  Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6.

4.  Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24.

(For complete reference list see EL-AH1 Certificate of Analysis)