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产品分类 > 食物过敏原 > Ara h 3 ELISA kit 花生过敏原Ara h3检测试剂盒

Ara h 3 ELISA kit 花生过敏原Ara h3检测试剂盒

产品货号:EL-AH3
促销: 12720.00
价格: 12720.00

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kit
美国Indoor
ELISA kit for measuring Arachis hypogaea allergen, Ara h 3.

Antibodies:1E8/4G9

Sensitivity:0.2ng/ml

Limit of Detection:2.0ng/ml

Assay Performance:
Contents:

Monoclonal antibody 1E8, Ara h 3 Allergen Standard, Biotinylated
antibody 4G9; sufficient reagents for 10 microtiter plates.

Storage:Store antibodies at 4°C; store ST-AH3 at -20°C upon arrival.

Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6

Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20

(PBS-T)

Bovine serum albumin

Streptavidin-Peroxidase

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:
Product Resources:EL-AH3 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.


Certificate of Analysis

Monoclonal Antibody:1E8

Vial:Red Cap; 100µl

Immunogen:Ara h 3

Isotype:Mouse IgG1

Specificity:Binds to species specific epitope present on Arachis

hypogaea
 allergen, Ara h 3.

Purification:Produced in vitro and purified by affinity chromatography using

Protein A. Single heavy and light chain bands on SDS-PAGE.

Concentration:1.1 mg/ml in phosphate buffered saline, pH 7.4.  Based on A280 for

IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.



Certificate of Analysis

Monoclonal Antibody:4G9

Vial:Brown Cap; 100µl

Immunogen:Ara h 3

Isotype:Mouse IgG1

Specificity:Binds to species specific epitope present on Arachis

hypogaea
 allergen, Ara h 3.

Purification:Produced in vitro and purified by affinity chromatography using

Protein A. Single heavy and light chain bands on SDS-PAGE.

Biotinylation:Biotinylated and titrated for use in ELISA at 1/1000 dilution.

Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered,

preservative free.


Certificate of Analysis

Allergen Standard:nAra h 3

Vial:White Cap; 400µl

Composition:Naturally purified Ara h 3 prepared in 1% BSA/50% glycerol/PBS,

pH 7.4.
Concentration:1,250ng/ml Ara h 3

Calibration:The Ara h 3 concentration of the purified Ara h 3 was determined by

amino acid analysis.

Stability/Storage:Please store the standard at -20ºC (+/- 5°C).


References:

1.  Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6.

2.  Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24.

(For complete reference list see EL-AH3 Certificate of Analysis)