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产品分类 > 皮屑毛发羽毛过敏原 > Fel d 1 ELISA kit (EL-FD1)

Fel d 1 ELISA kit (EL-FD1)

产品货号:EL-FD1
促销: 12720.00
价格: 12720.00

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kit
美国Indoor
ELISA kit for measuring Felis domesticus allergen, Fel d 1.

Antibodies:6F9/3E4

Sensitivity:0.2ng/ml

Limit of Detection:0.8ng/ml

Assay Performance:Intra-assay CV% of 8.3; Inter-assay CV% of 19

Contents:

Monoclonal antibody 6F9, Universal Allergen Standard,

Biotinylated antibody 3E4; sufficient reagents for 10 microtiter

plates.

Storage:Store at 4°C

Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6

Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20

(PBS-T)

Bovine serum albumin

Streptavidin-Peroxidase

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:
Product Resources:

EL-FD1 Certificate of Analysis

Focus on... Fel d 1

For research and commercial use in vitro: not for human in vivo or therapeutic use.


Certificate of Analysis

Monoclonal Antibody:6F9 (clone 6F9 A4 H1)

Vial:Red Cap; 100µl

Immunogen:Fel d 1

Isotype:Mouse IgG1

Specificity:

Binds to an epitope on cat Felis domesticus allergen, Fel d 1,

Chain 1.

Purification:Produced in tissue culture and purified by affinity

chromatography using recombinant Protein G. Single heavy and

light chain bands on SDS-PAGE.

Concentration:1.0 mg/ml in phosphate buffered saline, pH 7.4. Based on A280

for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.



Certificate of Analysis

Monoclonal Antibody:3E4 (clone 3E4 C4 C10)

Vial:Brown Cap; 100µl

Immunogen:Fel d 1

Isotype:Mouse IgG1

Specificity:Binds to an epitope on cat Felis domesticus allergen, Fel d 1,

Chain 1.

Purification:Produced in ascites and purified by ammonium sulphate

precipitation and HPLC using recombinant Protein G. Single

heavy and light chain bands on SDS-PAGE.

Biotinylation:Biotinylated and titrated for use in ELISA for Fel d 1 allergen at

1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH

7.4, 0.22µm filtered, preservative free.



Certificate of Analysis

Allergen Standard:Universal Allergen Standard

Vial:White Cap; 400µl

Composition:A formulation of eight purified natural allergens prepared

in 1% BSA/50% glycerol/PBS, pH 7.4

Concentration/Calibration:
Universal Allergen Standard

Protein Measurement

Concentration (ng/ml)

Der p 1

Amino-acid analysis

2500

Der f 1

Amino-acid analysis

2500

Der p 2

Amino-acid analysis

1000

Fel d 1

Amino-acid analysis

1000

Can f 1

Amino-acid analysis

2500

Rat n 1

Amino-acid analysis

1000

Mus m 1

Amino-acid analysis

250

Bla g 2

Amino-acid analysis

2500

References:

1.  Chapman MD, Aalberse RC, Brown MJ, Platts-Mills TA. Monoclonal antibodies to the major feline allergen Fel d 1. 11. Single step affinity purification of Fel d 1, N terminal sequence analysis, and development of a sensitive two-site immunoassay to assess Fel d 1 exposure. J Immunol 1988; 140(3):812-818.

2.  de Blay F, Chapman MD, Platts-Mills TA. Airborne cat allergen (Fel d 1). Environmental control with the cat in situ. Am Rev Respir Dis 1991; 143:1334-39.

3.  Ingram JM, Sporik R, Rose G, Honsinger R, Chapman MD, Platts-Mills TA. Quantitative assessment of exposure to dog (Can f 1) and cat (Fel d 1) allergens: relation to sensitization and asthma among children living in Los Alamos, New Mexico. J Allergy Clin Immunol 1995; 96(4):449-456.

4.  Patchett K, Lewis S, Crane J, Fitzharris P. Cat Allergen (Fel d 1) levels on school children’s clothing and in primary school classrooms in Wellington, New Zealand. J Allergy Clin Immunol 1997; 100(6 Pt ):755-759

5.  Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoor allergens and asthma: report of the Third International Workshop. J Allergy Clin Immunol 1997; 100(6 Pt 1):S2-24.

6.  Platts-Mills TA, Vaughan J, Squillace S, Woodfolk J, Sporik R. Sensitisation, asthma, and a modified Th2 response in children exposed to cat allergen: a population-based cross-sectional study. Lancet 2001; 357(9258):752-756.

7.  Arbes SJ, Jr.,Cohn RD, Yin M, Muilenberg ML, Friedman W, Zeldin DC. Dog allergen (Can f 1) and cat allergen (Fel d 1) in US homes: results from the National Survey of Lead and Allergens in Housing. J Allergy Clin Immunol 2004; 114(1):111- 117.

8.  Heinrich J, Bedada GB, Zock JP, Chinn S, Norback D, Oliveri M et al. Cat allergen level: its determinants and relationships to specific IgE to cat across European centers. J Allergy Clin Immunol 2006; 118(3):674-681

9.  Jarvis D, Zock JP, Heinrich J, Svanes C, Verlato G, Olivieri M et al. Cat and dust mite allergen levels, specific IgG and IgG(4), and respiratory symptoms in adults. J Allergy Clin Immunol. 2007 Mar; 119(3):697-704.

(For complete reference list see EL-FD1 Certificate of Analysis)