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产品分类 > 皮屑毛发羽毛过敏原 > Rat n 1 ELISA kit (EL-RN1)

Rat n 1 ELISA kit (EL-RN1)

产品货号:EL-RN1
促销: 12720.00
价格: 12720.00

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kit
美国Indoor

ELISA kit for measuring Rat Urinary Protein (RUP) allergen, Rat n 1.

Antibodies:RUP-6/RUP-1

Sensitivity:0.2ng/ml

Limit of Detection:0.8ng/ml

Assay Performance:Inquire

Contents:

Monoclonal antibody RUP-6, Universal Allergen Standard,

Biotinylated antibody RUP-1; sufficient reagents for 10

microtiter plates.

Storage:Store at 4°C

Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6

Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin

Streptavidin-Peroxidase

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:
Product Resources:EL-RN1 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.


Certificate of Analysis

Monoclonal Antibody:RUP-6

Vial:Red Cap; 100µl

Immunogen:Rat n 1

Isotype:Mouse IgG1

Specificity:Binds to an epitope on rat Rattus norvegicusurinary

allergen, Rat n 1.

Purification:Produced in cell culture and purified by affinity

chromatography using Protein G.

Concentration:1.0mg/ml in phosphate buffered saline, pH 7.4. Based on

A280 for IgG (1.42=1mg/ml), 0.22μm filtered, 0.02%

sodium azide.


Certificate of Analysis

Biotinylated Antibody:RUP-1

Vial:Brown Cap; 100µl

Immunogen:Rat n 1

Isotype:Mouse IgG1

Specificity:

Binds to an epitope on rat Rattus norvegicus urinary

allergen, Rat n 1.

Purification:Produced in cell culture and purified by affinity

chromatography using Protein G.

Concentration:1.0mg/ml in phosphate buffered saline. Based on A280 for

IgG (1.42= 1mg/ml), 0.22µm filtered, 0.02% sodium azide.

Biontinylation:Biotinylated and titrated for use in ELISA at 1/1000

dilution.



Certificate of Analysis

Allergen Standard:Universal Allergen Standard

Vial:White Cap; 400µl/vial

Composition:A formulation of eight purified natural allergens prepared in

1% BSA/50% glycerol/PBS, pH 7.4.

Concentration/Calibration:


Universal Allergen Standard

Protein Measurement

Concentration (ng/ml)

Der p 1

Amino-acid analysis

2500

Der f 1

Amino-acid analysis

2500

Der p 2

Amino-acid analysis

1000

Fel d 1

Amino-acid analysis

1000

Can f 1

Amino-acid analysis

2500

Rat n 1

Amino-acid analysis

1000

Mus m 1

Amino-acid analysis

250

Bla g 2

Amino-acid analysis

2500

References:

1.  Renstrom A, Larsson PH, Malmberg P, Bayard C. A new amplified monoclonal rat allergen assay used for evaluation of ventilation improvements in animal rooms. J Allergy Clin Immunol. 1997 Nov;100(5):649-55.

2.  Renstrom A, Gordon S, Larsson PH, Tee RD, Newman Taylor AJ, Malmberg P. Comparison of a radioallergosorbent (RAST) inhibition method and a monoclonal enzyme linked immunosorbent assay (ELISA) for aeroallergen measurement. Clin Exp Allergy. 1997 Nov;27(11):1314-21.

(For complete reference list see EL-RN1 Certificate of Analysis)