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产品分类 > 血栓栓塞综合征 > β2-Glycoprotein 1 (Apo H; non recombinant; human)/β2糖蛋白1(载脂蛋白H;非重组;人)

β2-Glycoprotein 1 (Apo H; non recombinant; human)/β2糖蛋白1(载脂蛋白H;非重组;人)

产品货号:11300/11301
促销: 0.00

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0.1mg 1mg
德国Diarect

Product Number: 11300
Description:
Human β2-glycoprotein 1 (β2-GP 1), also known as Apolipoprotein H or Apo H. Natural antigen for solid (ELISA) and fluid phase diagnostic assays.
Immunological function:
Binds IgG, IgA and IgM-type human auto-antibodies. Auto-antibodies to β2-glycoprotein 1 recognize conformation-dependent and linear epitopes.
Origin:
Native. Purified from human serum by protein-chemical methods.
Biochemical tests:
SDS-PAGE (purity > 90 %); Western blot with polyclonal rabbit anti-ApoH antibody.
Observed molecular weight (SDS-PAGE):
Approx. 62 kDa
Immunological tests/Functionality: Standard ELISA test (checkerboard analysis of positive/negative sera panels).
Recommended buffer/storage and handling conditions:
Recommendations for storage buffer: ionic strength around 250 mM, neutral to slightly alkaline pH and 20 % glycerol as cryoprotective agent. Storage temperature: –70° to –80° C. Repeated freeze/thaw cycles should be avoided. Use of reducing sulfhydryl reagents and denaturing detergents will result in the destruction of conformational epitopes.
Coating concentration:
0.5-1.0 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
Due to the diversity of existing β2-glycoprotein 1 / ApoH coating protocols (e.g. binding to phospholipid-precoated plates) further recommendations cannot be given.