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产品分类 > 过敏疾病检测原料 > Recombinant Bla g 2 (RP-BG2-1)

Recombinant Bla g 2 (RP-BG2-1)

产品货号:RP-BG2-1
促销: 8720.00
价格: 8720.00

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250ug
美国Indoor
Allergen:rBla g 2 (Blattella germanica allergen 2 )

Unit:250µg

Source:P. pastoris (clone NIG rBla g 2, glycosylation site N93Q mutated)

Mol. Wt:~37 kD

Purification:Purified from Pichia pastoris culture supernatant by monoclonal antibody affinity chromatography. Purity by silver stained SDS-PAGE > 95%.

Concentration:

See product insert. 

Formulation:

Preservative and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22 µm filter.

Storage:Store at -20ºC.

Notes:(1) One glycosylation site was removed in this recombinant leaving two glycosylation sites. The recombinant is glycosylated and has a slightly higher MW than natural. Bla g 2 (36kD).

(2) This recombinant form was used to determine the crystal structure of Bla g 2.

Genbank:U28863 Mutation N93Q, substituted cag (Q) for aac (N)

PDB:1YG92NR6 (with Fab-7C11), X-ray crystal structures

Product Resources:Recombinant Bla g 2 Certificate of Analysis

Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.

References:

  1. Arruda LK, Vailes LD, Mann BJ, Shannon J, Fox JW, Vedvick TS, Hayden ML, Chapman MD. Molecular Cloning of a major cockroach (Blatella germanica) allergen, Bla g 2: Sequence homology to the aspartic proteases. J Biol Chem 1995;270:19563-8.

  2. Wünschmann S, Gustchina A, Chapman MD, Pomés A. Cockroach allergen Bla g 2: An unusual aspartic proteinase. J Allergy Clin Immunol 2005;116:140-5.

  3. Gustchina A, Li M, Wünschmann S, Chapman MD, Pomés A, Wodawer A. Crystal structure of cockroach allergen Bla g 2, an unusual zinc binding aspartic protease with a novel mode of self-inhibition. J Mol Biol 2005;348:433-44.

  4. Pomés A, Chapman MD, Vailes LD, Blundell TL, Venugopal D. Cockroach allergen Bla g 2: Structure function and implication for allergic sensitization. Amer J Resp Crit Care Med 2002;165:391-7.

  5. Li M, Gustchina A, Alexandratos J, Wlodawer A, Wünschmann S, Kepley CL, Chapman MD, Pomés A. Crystal structure of a dimerized cockroach allergen Bla g 2 complexed with a monoclonal antibody. J Biol Chem, 2008;283:22806-22814