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Recombinant Der p 1 deglycosylated 重组去糖基化屋尘螨过敏原Der p 1 deglycosylated

产品分类 > 过敏疾病检测原料 > Recombinant Der p 1 deglycosylated 重组去糖基化屋尘螨过敏原Der p 1 deglycosylated

Recombinant Der p 1 deglycosylated 重组去糖基化屋尘螨过敏原Der p 1 deglycosylated

产品货号:RP-DP1D-1
促销: 9520.00
价格: 9520.00
分享到:
250ug
美国Indoor
Allergen:rDer p 1 (Dermatophagoides pteronyssinus allergen 1) deglycosylated

Unit:250µg

Source:Pichia pastoris (clone N52Q, N-glycosylation site N52 mutated)

Mol. Wt:24 kD

Purification:

Purified from Pichia pastoris culture by multi-step column

chromatography. Purity on silver stained SDS-PAGE >95%.

Concentration:

See product insert. 

Formulation:

Preservative and carrier-free in sterile, 0.1M CAPS Buffer, pH 9.5. Filtered through 0.22 µm filter.

Storage:Store at -20ºC. Avoid freeze-thaw cycles.

Notes:1. A LoTox™ product: Endotoxin ≤ 0.03 EU/µg.

2. Mature form of rDer p 1; proregion removed with acid dialysis.

3. Cysteine protease activity (DTT dependent):

(see product insert) RFU @ 0.25µg/ml.

4. For stability purposes the product is shipped in CAPS Buffer pH9.5. It may be dialysed or diluted into a physiological buffer for cell culture purposes, but long term storage in PBS may cause

precipitation.

Genbank:U11695 = pro-Der p 1.0102 (difference is that in pro Der p 1.0105, V124 becomes A124)

PDB:1XKG, 2AS83F5V X-ray crystal structures

Product Resources:Recombinant Der p 1 DG Certificate of Analysis

Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.

References:


  1. Hewitt CRA, Brown AP, Hart BJ, Pritchard DL. A major house dust mite allergen disrupts the Immunoglobulin E network by selectively cleaving CD23: innate protection by antiproteases. J Exp Med 1995;182:1537-1544.

  2. Schultz O, Laing P, Sewell HF, Shakib F. Der p 1, a major allergen of the house dust mite, proteolytically cleaves the low affinity receptor for human IgE (CD23). Eur J Immunol 1995;25:3191-3194.

  3. King C, Brennan S, Thompson PJ, Stewart GA. Dust mite proteolytic allergens induce cytokine release from cultured airway epithelium. J Immunol 1998; 161:3645-3651.

  4. Wan H, Winton HL, Soeller C, Tovey ER, Gruenert DC, Thompson PJ, Stewart GA, Taylor GW, Garrod DR, Cannell MD, Robinson C. Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions. J Clin Invest 1999; 104:123-133.

  5. Best BE, Stedman KE, Bozic CM, Hunter SW, Vailes LD, Chapman MD, McCall CA, McDermott MJ. A recombinant group 1 house dust mite allergen, rDer f 1, with biological activities similar to those of the native allergen. Prot Exp Pur 2000;20:462-71.

  6. Chruszcz M, Chapman MD, Vailes LD, Stura EA, Saint-Remy JM, Minor W, Pomés A. Crystal structures of mite allergens Der f 1 and Der p 1 reveal differences in surface-exposed residues that may influence antibody binding. J Mol Biol, 2009;386:520-530.