LoTox™ Natural Der p 1 屋尘螨过敏原Der p 1

From mite culture by multi-step chromatographic purification.

Purity on silver stained SDS-PAGE >95%.

Preservative and carrier-free in sterile, endotoxin-free,

phosphate buffered saline, pH 7.4

LoTox™ Natural Der p 1 Certificate of Analysis

1. Lombardero M, Heymann PW, Platts-Mills TAE, Fox JW, Chapman MD. Conformational stability of B cell epitopes on Group 1 and Group II Dermatophagoides spp. Allergens. Effect of thermal and chemical denaturation on the binding of murine IgG and human IgE antibodies. J Immunol 1990;144:1353-1360.

2. Hewitt CRA, Brown AP, Hart BJ, Pritchard DL. A major house dust mite allergen disrupts the Immunoglobulin E network by selectively cleaving CD23: innate protection by antiproteases. J Exp Med 1995;182:1537-1544.

3. Schultz O, Laing P, Sewell HF, Shakib F. Der p 1, a major allergen of the house dust mite, proteolytically cleaves the low affinity receptor for human IgE (CD23). Eur J Immunol 1995;25:3191-3194.

4. King C, Brennan S, Thompson PJ, Stewart GA. Dust mite proteolytic allergens induce cytokine release from cultured airway epithelium. J Immunol 1998; 161:3645-3651.

5. Gough L, Schultz O, Sewell HF, Shakib F. The cysteine protease activity of the major house dust mite allergen Der p 1 selectively enhances the immunoglobulin E antibody responses. J Exp Med 1999; 190:1897-1901.

6. Wan H, Winton HL, Soeller C, Tovey ER, Gruenert DC, Thompson PJ, Stewart GA, Taylor GW, Garrod DR, Cannell MD, Robinson C. Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions. J Clin Invest 1999; 104:123-133.

7. Chruszcz M, Chapman MD, Vailes LD, Stura EA, Saint-Remy JM, Minor W, Pomés A. Crystal structures of mite allergens Der f 1 and Der p 1 reveal differences in surface-exposed residues that may influence antibody binding. J Mol Biol, 2009;386:520-530.