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产品分类 > 花粉过敏原 > Bet v 1 ELISA kit 白桦过敏原Bet v 1检测试剂盒

Bet v 1 ELISA kit 白桦过敏原Bet v 1检测试剂盒

产品货号:EL-BV1
促销: 12720.00
价格: 12720.00

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kit
美国Indoor
ELISA kit for measuring Betula verrucosa allergen, Bet v 1.

Antibodies:4B10/2E10

Sensitivity:0.2ng/ml

Limit of Detection:3.1ng/ml

Assay Performance:Inquire

Contents:

Monoclonal antibody 4B10, Bet v 1 Standard, Biotinylated antibody 2E10; sufficient reagents for 10 plates.

Storage:Store at 4°C.

Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6

Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)

Bovine Serum Albumin

Streptavidin-Peroxidase

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:
Product Resources:

EL-BV1 Certificate of Analysis

Bet v 1 Standard Letter - October 2017

For research and commercial use in vitro: not for human in vivo or therapeutic use.


Certificate of Analysis

Monoclonal Antibody:4B10 (clone 4B10)

Vial:Red Cap; 100µl

Immunogen:Bet v 1

Isotype:Mouse IgG1

Specificity:Binds to multiple isoforms of Betula verrucosa allergen, Bet v 1.

Purification:Protein A affinity chromatography

Concentration:1mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free. 



Certificate of Analysis

Monoclonal Antibody:2E10 (clone 2E10 G6 G7)

Vial:Brown Cap; 100µl

Immunogen:Bet v 1

Isotype:Mouse IgG1

Specificity:Binds to a specific epitope on Betula verrucosa allergen, Bet v 1.

Purification:Enrichment and concentration using 50% saturated ammonium sulphate precipitation followed by purification using protein A affinity chromatography.

Biotinylation:Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free. 



Certificate of Analysis

Allergen Standard:rBet v 1a

Vial:White Cap; 400µl

Composition:Recombinant Bet v 1a prepared in 1% BSA/50 glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4.

Concentration:1000ng/ml Bet v 1


Calibration:The rBet v 1a was produced from Pichia and purified by a multi-step chromatography procedure. Validity of the rBet v 1 standard was confirmed by comparison with the European Pharmacopoeia reference CRS

(Y0001565), recombinant major allergen rBet v 1 containing 10.25μg of rBet v 1 per vial. 

References:


1. Breiteneder H, Pettenburger K, Bito A, et al. The gene coding for themajor birch pollen allergen Bet v 1, is highly homologous to a peadisease resistance response gene. EMBO J. 1989;8:1935-8. 

2. Hoffmann-Sommergruber K, Susani M, Ferreira F, et al. High-levelexpression and purification of the major birch pollen allergen, Bet v 1.Protein Expr Purif. 1997;9:33-9. 

3. Godnic-Cvar J, Susani M, Breiteneder H, et al. Recombinant Bet v 1, themajor birch pollen allergen, induces hypersensitivity reactions equal tothose induced by natural Bet v 1 in the airways of patients allergic to treepollen. J Allergy Clin Immu nol. 1997;99:354-9. 

4. Schappi GF, Monn C, Wuthrich B, Wanner HU. Direct determination ofallergens in ambient aerosols: methodological aspects. Int Arch AllergyImmunol. 1996;110:364-70. 

5. Schappi GF, Suphioglu C, Taylor PE, Knox RB. Concentrations of themajor birch tree allergen Bet v 1 in pollen and respirable fine particles inthe atmosphere. J Allergy Clin Immunol. 1997;100:656-61. 

6. S. Vieths, D. Barber, M. Chapman, et al. Establishment of recombinantmajor allergens Bet v 1 and Phl p 5a as Ph. Eur. Reference standardsand validation of ELISA methods for their measurement. Pharmeuropa Bio&SN October 2012;118-13. 

(For complete reference list see EL-BV1 Certificate of Analysis)